Injecting variable volumes using the partial loop - fi ll method with the Agilent 1260 Infi nity Analytical SFC System

The Agilent 1260 Infi nity Analytical Supercritical Fluid Chromatography (SFC) System provides LC-like sensitivity and UHPLC power range. It is a pressurized system that has a fi xed-loop autosampler. The injection loops come in different sizes to perform fi xed-loop injections. Injecting a volume smaller than the loop volume is possible using the partial loop-fi ll injection method where the loop is fi lled only partially with sample. This Technical Overview shows the usability of the partial loop-fi ll method to inject variable volumes. The method provides area and retention time RSD (%) of less than 3.5% and 0.1% respectively and results in a linear response for increasing injector volumes. Authors Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India Vinayak AK Amrita School of Biotechnology Amrita Vishwa Vidyapeetham Kollam, India